Description
Vit Kit – Warm NX is a versatile and affordable system designed for warming oocytes and embryos. Unlike typical vitrification kits with a mono-buffered system and M199 base medium for chick embryo fibroblasts, Vit Kit – Warm NX uses IVF media optimized for human embryo culture.
Features:
- Based on CSCM and MHM to reduce stress on oocytes and embryos, ensuring high survival rates
- Dual-buffered (HEPES and MOPS) for a stable pH environment
- Trehalose as a non-permeating cryoprotectant for enhanced membrane stabilization
- Supplemented with 20% (v/v) DSS for a final concentration of 10 mg/mL HSA and 4 mg/mL Dextran
- Each kit can be used for up to 12 warming applications with embryos or oocytes
Product Number:
90183
Configuration:
Thawing NX-TS (yellow cap) 6x2mL Vials
Dilaution NX-DS (orange cap) 2x1mL Vials
Washing NX-WS (red cap) 4x1mL Vials
Each kit can be used for up to 12 warming applications with embryos or oocytes.
Details
Vit Kit – Warm NX is an adaptable, cost-effective system for use in the thawing of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Unlike many vitrification kits that feature a mono-buffered system and M199 base medium originally formulated for chick embryo fibroblasts, Vit Kit – Warm NX is derived from current IVF media tailored specifically for human embryo culture.
The backbone of Vit Kit – Warm NX is Continuous Single Culture medium, an IVF medium intended for conventional fertilization and culture from Day 1 and beyond. Similar to our Multipurpose Handling Medium, Vit Kit-NX takes advantage of two buffers, HEPES and MOPS, to provide a more secure pH environment for oocytes and embryos during vitrification. The integration of these key ingredients delivers consistent results, including high oocyte and embryo survival rates, while also offering an effective, flexible, and economical media ideally suited for the nuanced needs of the modern IVF laboratory.
Versatile Formulas Deliver Consistent Survival Rates Across Different Vitrification Media
Vit Kit – Warm NX was capable of warming human oocytes frozen in Vit Kit (A) and a top competitor’s vitrification media (B).
Previously frozen MII oocytes were re-frozen in different vitrification media with the Cryolock device. MII oocytes were counted towards the survival rate and were recovered in CSCM-NXC (PN 90168) for the immediate and 2h post-thaw time points. Study was performed in collaboration with World Egg Bank in Phoenix, Arizona, USA.
Specifications
Shelf Life
12 months from date of manufacture
If you want to hear more about the product or place an order, you can contact us at: info@novivitae.com
